I didn't know whether I should put this in the anti-oxidant section or here..but I found this an interesting read...on COQ10 nanoemulsions:
http://www.in-cosmeticsasia.com/ExhibitorLibrary/59/Preparation_and_Properties_of_Coenzyme_Q10_Nanoemulsions_Cosmetic_Science_Technologie_2006_3.pdfUse of CoQ10 nanoemulsions in cosmetics
CoQ10 encapsulated in the described nanoemulsions increases the synthesis of collagen I in fibroblasts. This effect was recently shown using normal human dermal fibroblasts (NHDF) and a CoQ10 nanoemulsion at a concentration of 0.1%. Cells first were cultured at standard conditions without CoQ10 during
24 hours. Then the CoQ10 nanoemulsion was added and cells were incubated for 72 hours.
The effect of CoQ10 was evaluated by visualization of the protein using a polyclonal antibody anti-collagen I and a fluorescent second antibody anti-immunglobuline-FITC. Results were photographed applying microscopy observation.
The photographs show an increased secretion of collagen I compared to the control. (figure 7)
This result demonstrates that CoQ10 encapsulated in nanodroplets positively influences the expression of collagen I by fibroblasts.
In a second in vitro assay the influence of CoQ10 on the activity of mitochondrial dehydrogenase in keratinocytes was assessed. Cells (human adult low calcium high temperature cells, HaCaT) were cultured according to standard procedures and incubated for 72 hours with 0.1% of a CoQ10 nanoemulsion. In a further step cells were incubated with sodium dodecylsulfate (SDS, 2?g/ml) for 24 hours to stress the cells.
The mitochondrial dehydrogenase activity was analyzed using the MTT test. The application of the CoQ10 nanoemulsion enhanced the activity of the unstressed keratinocytes to 116.8% +/-3.2% compared to the control 100.0% +/-1.3%. The SDS treatment decreased the cell activity to 67.1% +/-4.1%.
Pre-treatment with encapsulated CoQ10 downsized the damageing effect of SDS and the cell activity analyzed by the MTT test only decreased to 80.2% +/-1.6%. (figure
The assessment reveals that CoQ10 nanoemulsions enhance the mitochondrial activity of keratinocytes and protect them against necrotic stress factors.
Since CoQ10 has a low bioavailability, strong endeavours have been made to develop efficient delivery systems. Latest research established the encapsulation of CoQ10 in nanoemulsions. Data show that the CoQ10 bioavailability is significantly enhanced by using nanoemulsions. This results in much higher CoQ10 serum levels after oral application which is of great importance for the treatment of different diseases.
The application of CoQ10 has been further improved by the development of novel CoQ10 double nanoemulsions containing tocopherol and CoQ10 in individual nanodroplets. In addition the CoQ10 concentration in these nanoemulsions could be increased by the development of a supersaturated CoQ10 nanoemulsion.
Cell Culture studies based on skin fibroblasts and keratinocytes using these novel CoQ10 nanoemulsions revealed that encapsulated CoQ10 supports the secretion of collagen I and stimulates the mitochondrial cell activity. In addition a significant protection against necrotic stress factors could also be shown
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